Arsenal have been handed a trip to Olympiacos in their first leg before welcoming the Greek team back to London.
It will be a chance of revenge for the Gunners after the Greeks beat them in the round of 32 last season.
Man Utd play AC Milan in a real heavyweight contest for this tournament. The last time the sides met was back in 2010 when United beat Milan home and away in the last 16 of the Champions League. A 3-2 win in Italy was followed up by a 4-0 thrashing at Old Trafford.
Spurs play Dinamo Zagreb and Rangers meet Slavia Prague, who knocked out Leicester City in the last round. The draw was made on Friday afternoon in Nyon, Switzerland at the House of European Football.
There were no seedings in the draw and nothing to stop clubs from the same country being drawn together. The ties are played over 11 and 18 March, with the draws for the quarter and semi-finals held on 19 March.
The full fixture list, with times and dates, for the last 16 round will come out later on Friday afternoon.
Europa League last 16 draw
- Ajax vs Young Boys
- Dynamo Kiev vs Villarreal
- Roma vs Shakhtar Donetsk
- Olympiacos vs Arsenal
- Dinamo Zagreb vs Tottenham Hotspur
- Man Utd vs AC Milan
- Slavia Prague vs Rangers
- Granada vs Molde
Tottenham manager Jose Mourinho was bullish about his side’s chances in the competition after knocking out Wolfsberger on Wednesday. ‘My record is good,’ he said. ‘I played the competition twice, I got to the final twice, I won twice. In the UEFA Europa League I was never knocked out.’
Rangers continued their impressive run in the competition on Thursday night as they despatched Royal Antwerp 5-2 at Ibrox to win 9-5 on aggregate.
Steven Gerrard realises that at the last 16 of the Europa League, the Scottish Premiership leaders are going to have it tough, commenting after the win on Thursday.
‘Whoever you come up against in the last 16, it is going to be a big challenge, especially if you get one of the English teams,’ said Gerrard. ‘We would be punching well above our weight.’
Former Rangers star Peter Lovenkrands said he would like to have seen his side Rangers ‘get an English team to see where the two leagues are against each other.’